N-acetyl Cysteine N-acetyl Cysteine Inhibits Exercise-induced Lymphocyte Apoptotic Protein Alterations
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چکیده
PURPOSE: To investigate the effect of strenuous exercise and antioxidant administration on proand antiapoptotic protein expression in intestinal lymphocytes. METHODS: Female C57BL/6 mice (N = 52) were randomly assigned to receive N-acetyl cysteine (NAC; 1 g.kg(-1)) or saline (SAL) 30 min before treadmill exercise (EX) for 90 min and 2 degrees slope (30 min at 22 m.min(-1), 30 min at 25 m.min(-1), and 30 min at 28 m.min(-1)) and sacrificed immediately (Imm) or 24 h (24 h) after exercise. Control mice were exposed to treadmill noise and vibration without running (nonexercised). Intestinal lymphocytes (IL) were isolated and proand antiapoptotic protein expression was evaluated by Western blot analysis. RESULTS: IL protein levels of proapoptotic (caspase 3 and cytosolic cytochrome c) and antiapoptotic (Bcl-2) were significantly different among groups. Relative to nonexercised mice, protein levels of caspase 3 (P < 0.001) and cytosolic cytochrome c (P < 0.005) were significantly elevated, whereas Bcl-2 (P < 0.05) was significantly lower immediately after exercise in mice receiving saline (EX + SAL + Imm) but not in animals receiving NAC (EX + NAC + Imm) or both 24 h postgroups (EX + SAL + 24 h and EX + NAC + 24 h). CONCLUSION: These results suggest that oxidative stress acting through a mitochondrial pathway may play a role in intestinal lymphocyte apoptosis after strenuous exercise.
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